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Current Protocols is a series of laboratory manuals for life scientists. The first title, Current Protocols in Molecular Biology, was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David D. Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of
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Protocols. Molecular Biology Procedures (Ambros Lab) A collection of protocols in molecular biology in a single page. Major topics include General Techniques for Handling Nucleic Acids, DEP treatment of solutions, Phenol Extraction, Ethanol Precipitation of Nucleic Acids, PEG Precipitation of DNA, Restriction Enzyme Digestion, RNA mRNA
Include prestained or biotinylated proteinmolecular weight standards in one or more gel lanes.The protein markers will transfer to the membrane and conveniently indicate membraneorientation and sizes of proteins after immunostaining.Table 10.8.1 Protein Standards for ImmunoblottingProtein standard ApplicationUnstained Molecular weight calibration and transfer efficiency; can be visualizedwith
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molecular biology for newcomers to the field, particularly hematolo- gists. Those wishing actually to introduce this methodology into their labs would perhaps be better served by one of the many excel- lent laboratory manuals that are currently available, particularly the second edition of Molecular Cloning: A Laboratory Manual (Sambrook, Fritsch and Maniatis) or Current Protocols in Molecular
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