Qiagen dna extraction protocol pdf

Qiagen dna extraction protocol pdf
High quality genomic DNA extraction using CTAB and Qiagen genomic-tip page1/4 Extraction of high quality DNA for Genome Sequencing Modified from protocols of Prof. Pietro Spanu (Imperial College, London) and T. M. Fulton, J. Chunwongse, S. D. Tanksley, Pl Mol Biol Rep 13, 207 (1995) Reagents required BUFFER A: 0.35 M sorbitol 0.1 M Tris-HCl, pH 9 5 mM EDTA, pH 8 BUFFER B: 0.2 M Tris …
DNA Protocols & Applications. Print Bookmark Share DNA extraction kits are available from many suppliers. The characteristics of the 3 most common types of DNA extraction kit are shown in the table Characteristics of common DNA extraction kits. Characteristics of common DNA extraction technologies : Anion-exchange: Silica-membrane technology: Magnetic-particle technology; What it …
The Wizard® Genomic DNA Purification Kit is designed for isolation of DNA from white blood cells (Sections 3.A, B and C), tissue culture cells and animal tissue (Section 3.D), plant tissue (Section 3.E), yeast (Section 3.F), and
For efficient, high-throughput, manual or automated purification of total cellular DNA from plant tissue Show details See products
Article (PDF Available) we developed a protocol for DNA extraction from sperm and semen fluid. The quality of extracted DNA was checked running on agarose gel, used for bisulfite conversion
A simple and rapid DNA extraction protocol capable of obtaining high-quality and quantity DNA from a large number of individuals is essential for assaying population and phylogenetic studies of plant pathogens. Most DNA extraction protocols used with oomycetes are …
20/11/2015 · While trying to find alternative DNA extraction protocols for improved DNA yield but without compromising the automation option, we found that a combination of tissue lysis protocol from Life Technologies and DNA extraction protocol from Qiagen yielded higher DNA amount than the protocol …
The ultrafast purification protocol, based on the remarkable selectivity of patented QIAGEN Resin, allows the isolation of ultrapure, supercoiled plasmid DNA with high yields in less than one hour, and without centrifugation.
Purification of DNA from stool 10 Purification of high-molecular-weight DNA 10 Processing of large-volume samples 10 High-throughput sample processing 10 The QIAamp Procedure 11 Amounts of starting material 11 Lysis with QIAGEN Protease or Proteinase K 12 Purification on QIAamp spin columns 12 Adsorption to the QIAamp membrane 12 Removal of residual contaminants 14 Elution of …
In the present study, a simple genomic DNA extraction protocol for different organ- isms is described, which is time- and cost-efficient, free of PCR-inhibiting contaminants, and not reliant on toxic reagents such as phenol/chloroform.
QIAGEN Supplementary Protocol Sample & Assay Technologies Automated purification of DNA from 8 or 16 samples of fresh or frozen whole blood on the
Protocol design: DNA extraction QIAGEN – McGill Food Safety & Quality Program 33. Sample & Assay Technologies PCR protocols on the QS AS Module Currently featured targets Featured PCR output formats Salmonella spp Listeria monocytogenes Listeria spp Campylobacter triple (jejuni, lari, coli) Campylobacter spp Cronobacter spp Rotor-Disc (for 72 Rotor-Disc rotor) Rotor Strips (for 72 Rotor …
The following protocol outlines the steps necessary to isolate total DNA from Gram-negative bacteria, and is a modified version of the Qiagen protocol that has been adopted by the Nelson Lab. Bacteria (maximum of 2.0 x 10 9 cells) are harvested by

QIAprep Spin Miniprep Columns QIAGEN

Easy and efficient protocol for oomycete DNA extraction
A. Standard DNA Purification Protocol 14 VI.Genomic DNA Isolation Systems 15 A. Solution-Based System 15 B. Silica Column-Based Systems 16 C. Cellulose Column-Based Systems 18 D. High-Throughput Genomic DNA Isolation Systems for Blood 19 E. Automated Low- to Moderate-Throughput for DNA Purification 20 F. Plant Genomic DNA Isolation 22 G. Food DNA Isolation 23 H. Fixed-Tissue Genomic DNA
QIAamp® DNA FFPE Tissue Handbook For purification of genomic DNA from formalin-fixed, paraffin-embedded tissues April 2010 Sample & Assay Technologies
A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots).
purification of total cellular DNA without organic extraction or ethanol precipitation. The buffer system is optimized to allow direct cell lysis followed by selective binding of DNA to the DNeasy membrane.
Purification of DNA from nematodes (PG40 Jun-10) page 2 of 3 Important points before starting In some steps of the procedure, one of 3 choices can be made.

Corrie Moreau Page 1 7/23/10 DNA Extractions using Qiagen DNeasy Kits with Extraction Beads Corrie Moreau – Field Museum (September 2009) These are the instructions I use for DNA extractions of individual or parts of ants, but are
QIAprep® Miniprep Handbook For purification of Plasmid DNA Large plasmids (>10 kb) Low-copy plasmids and cosmids Plasmid DNA prepared by other methods
PROTOCOL OF DNA EXTRACTION 1- Phenol/chloroform extraction and ethanol precipitation (INPBARCELONA) 1.1 Phenol extraction of Genomic DNA from frozen tissue 1.2 Phenol extraction of Genomic DNA from fixed paraffin-embedded tissue 1.3. Phenol extraction of Genomic DNA from formalin-fixed tissue or tissue treated with Formic acid 2- DNeasy® Tissue (Qiagen) 2.1 Purification of Genomic DNA …
QIAprep® Miniprep Handbook For purification of molecular biology grade DNA Plasmid Large plasmids (>10 kb) Low-copy plasmids and cosmids Plasmid DNA prepared by other methods
The following DNA extraction protocol is a combination of two Qiagen protocols for Disruption/Lysis of Tissue (Qiagen MagAttract HMW DNA Kit Handbook 08/2013, page 18) and Manual Purification of High-Molecular-Weight Genomic
QIAamp ® DNAMiniand BloodMiniHandbook For DNA purification from whole blood, plasma, serum, buffy coat, lymphocytes, dried blood spots (QIAamp DNA …
QIAamp® DNA Micro Handbook For purification of genomic DNA from small volumes of blood dried blood spots swabs forensic case work samples chewing gum
Stool DNA Extraction Procedure – December 2013 1 Stool It’s important to test beforehand the efficacy of this protocol on a few samples (not all of them at once), just in case you have to adjust your protocol (lysis time, elution volume,…). When you sample feces, it’s better to take the one of the extremities as it contains more cells. 1. DNA extraction 1.1.QIAamp DNA Stool Minikit
The MinElute DNA Cleanup system is specially designed for fast and easy isolation of DNA fragments from PCR reactions, agarose gels, or enzymatic reactions with …

In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
Technologies and the DNA extraction protocol from Qiagen yielded a higher DNA amount than the protocol using the Qiagen or Life Technologies kit only. We have not rigorously tested all the possible combinations of extraction
prior to DNA extraction on the QIAsymphony SP. DNA extraction DNA was extracted from a 1 mL aliquot of each Oragene saliva sample on the QIAsymphony SP using the QIAsymphony DSP DNA Midi Kit (QIAGEN® catalogue number: 937255 or 937236) and protocol Oragene_ID372_V2.xml. ˚ is protocol was speci˛ cally developed for use with Oragene saliva samples and includes a heated …
This protocol is for the purification of up to 10 μg DNA (70 bp to 10 kb). The yellow color of Buffer QG indicates a pH ≤7.5. DNA adsorption to the membrane is
protocols that do not include a lysis step. Alcohol precipitation is the precipitation of Alcohol precipitation is the precipitation of proteinase K-digested lysates after …
QIAGEN Supplementary Protocol AutoGen – Automated DNA
– protocol independent multicast tutorial

4. DNA Extraction and Quantification DNA Technologies Core

DNA Extraction Protocol Moreau Lab

Extraction of high quality DNA for Genome Sequencing

DNA Isolation DNeasy Blood & Tissue Kits Qiagen

–

Extraction of high quality DNA for Genome Sequencing
DNA isolation methods Chemistry – Faculty of Science

The Wizard® Genomic DNA Purification Kit is designed for isolation of DNA from white blood cells (Sections 3.A, B and C), tissue culture cells and animal tissue (Section 3.D), plant tissue (Section 3.E), yeast (Section 3.F), and
In the present study, a simple genomic DNA extraction protocol for different organ- isms is described, which is time- and cost-efficient, free of PCR-inhibiting contaminants, and not reliant on toxic reagents such as phenol/chloroform.
High quality genomic DNA extraction using CTAB and Qiagen genomic-tip page1/4 Extraction of high quality DNA for Genome Sequencing Modified from protocols of Prof. Pietro Spanu (Imperial College, London) and T. M. Fulton, J. Chunwongse, S. D. Tanksley, Pl Mol Biol Rep 13, 207 (1995) Reagents required BUFFER A: 0.35 M sorbitol 0.1 M Tris-HCl, pH 9 5 mM EDTA, pH 8 BUFFER B: 0.2 M Tris …
prior to DNA extraction on the QIAsymphony SP. DNA extraction DNA was extracted from a 1 mL aliquot of each Oragene saliva sample on the QIAsymphony SP using the QIAsymphony DSP DNA Midi Kit (QIAGEN® catalogue number: 937255 or 937236) and protocol Oragene_ID372_V2.xml. ˚ is protocol was speci˛ cally developed for use with Oragene saliva samples and includes a heated …
Technologies and the DNA extraction protocol from Qiagen yielded a higher DNA amount than the protocol using the Qiagen or Life Technologies kit only. We have not rigorously tested all the possible combinations of extraction

DNA isolation methods Chemistry – Faculty of Science
DNA Extraction Protocol Moreau Lab

purification of total cellular DNA without organic extraction or ethanol precipitation. The buffer system is optimized to allow direct cell lysis followed by selective binding of DNA to the DNeasy membrane.
QIAamp® DNA FFPE Tissue Handbook For purification of genomic DNA from formalin-fixed, paraffin-embedded tissues April 2010 Sample & Assay Technologies
QIAamp ® DNAMiniand BloodMiniHandbook For DNA purification from whole blood, plasma, serum, buffy coat, lymphocytes, dried blood spots (QIAamp DNA …
The following protocol outlines the steps necessary to isolate total DNA from Gram-negative bacteria, and is a modified version of the Qiagen protocol that has been adopted by the Nelson Lab. Bacteria (maximum of 2.0 x 10 9 cells) are harvested by

Extraction of high quality DNA for Genome Sequencing
Easy and efficient protocol for oomycete DNA extraction

For efficient, high-throughput, manual or automated purification of total cellular DNA from plant tissue Show details See products
Stool DNA Extraction Procedure – December 2013 1 Stool It’s important to test beforehand the efficacy of this protocol on a few samples (not all of them at once), just in case you have to adjust your protocol (lysis time, elution volume,…). When you sample feces, it’s better to take the one of the extremities as it contains more cells. 1. DNA extraction 1.1.QIAamp DNA Stool Minikit
purification of total cellular DNA without organic extraction or ethanol precipitation. The buffer system is optimized to allow direct cell lysis followed by selective binding of DNA to the DNeasy membrane.
Purification of DNA from nematodes (PG40 Jun-10) page 2 of 3 Important points before starting In some steps of the procedure, one of 3 choices can be made.
A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots).
In the present study, a simple genomic DNA extraction protocol for different organ- isms is described, which is time- and cost-efficient, free of PCR-inhibiting contaminants, and not reliant on toxic reagents such as phenol/chloroform.
QIAprep® Miniprep Handbook For purification of Plasmid DNA Large plasmids (>10 kb) Low-copy plasmids and cosmids Plasmid DNA prepared by other methods

QIAGEN Supplementary Protocol Purification of archive
Extraction of high quality DNA for Genome Sequencing

Purification of DNA from stool 10 Purification of high-molecular-weight DNA 10 Processing of large-volume samples 10 High-throughput sample processing 10 The QIAamp Procedure 11 Amounts of starting material 11 Lysis with QIAGEN Protease or Proteinase K 12 Purification on QIAamp spin columns 12 Adsorption to the QIAamp membrane 12 Removal of residual contaminants 14 Elution of …
QIAamp ® DNAMiniand BloodMiniHandbook For DNA purification from whole blood, plasma, serum, buffy coat, lymphocytes, dried blood spots (QIAamp DNA …
Corrie Moreau Page 1 7/23/10 DNA Extractions using Qiagen DNeasy Kits with Extraction Beads Corrie Moreau – Field Museum (September 2009) These are the instructions I use for DNA extractions of individual or parts of ants, but are
protocols that do not include a lysis step. Alcohol precipitation is the precipitation of Alcohol precipitation is the precipitation of proteinase K-digested lysates after …
QIAprep® Miniprep Handbook For purification of Plasmid DNA Large plasmids (>10 kb) Low-copy plasmids and cosmids Plasmid DNA prepared by other methods
High quality genomic DNA extraction using CTAB and Qiagen genomic-tip page1/4 Extraction of high quality DNA for Genome Sequencing Modified from protocols of Prof. Pietro Spanu (Imperial College, London) and T. M. Fulton, J. Chunwongse, S. D. Tanksley, Pl Mol Biol Rep 13, 207 (1995) Reagents required BUFFER A: 0.35 M sorbitol 0.1 M Tris-HCl, pH 9 5 mM EDTA, pH 8 BUFFER B: 0.2 M Tris …
In the present study, a simple genomic DNA extraction protocol for different organ- isms is described, which is time- and cost-efficient, free of PCR-inhibiting contaminants, and not reliant on toxic reagents such as phenol/chloroform.
A. Standard DNA Purification Protocol 14 VI.Genomic DNA Isolation Systems 15 A. Solution-Based System 15 B. Silica Column-Based Systems 16 C. Cellulose Column-Based Systems 18 D. High-Throughput Genomic DNA Isolation Systems for Blood 19 E. Automated Low- to Moderate-Throughput for DNA Purification 20 F. Plant Genomic DNA Isolation 22 G. Food DNA Isolation 23 H. Fixed-Tissue Genomic DNA
QIAamp® DNA FFPE Tissue Handbook For purification of genomic DNA from formalin-fixed, paraffin-embedded tissues April 2010 Sample & Assay Technologies
A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots).
Purification of DNA from nematodes (PG40 Jun-10) page 2 of 3 Important points before starting In some steps of the procedure, one of 3 choices can be made.
This protocol is for the purification of up to 10 μg DNA (70 bp to 10 kb). The yellow color of Buffer QG indicates a pH ≤7.5. DNA adsorption to the membrane is