Qiagen gel extraction kit protocol pdf

Qiagen gel extraction kit protocol pdf
the QIAamp® Viral RNA Mini Kit. support the extraction protocol as described below (70 µL input volume), an initial Qiagen Buffer AVL with carrier RNA is stable at …
18/11/2009 · One half was purified using four Qiagen gel extraction columns and the other half was purified using a single glass syringe filter as described above. The quantity of DNA purified using the glass syringe filter was equivalent to that purified by the four Qiagen gel extraction columns ( Figure. 5 ).
QIAGEN workstations and kits are intended as general-purpose devices. No claim or representation is intended for their use to i dentify any specific organ- No claim or representation is intended for their use to i dentify any specific organ-
DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s Gel Extraction Kit and a Company Q kit for gel extraction following manufacturer’s recommended protocols.
MinElute Gel Extraction Kit Protocols 19 using a microcentrifuge 19 using a vacuum manifold 21 MinElute Reaction Cleanup Kit Protocols 24 using a microcentrifuge 24 using a vacuum manifold 26 Troubleshooting Guide 28 Appendix: QIAvac Vacuum Manifolds 30 Handling Guidelines for QIAvac 24 30 Handling Guidelines for QIAvac 6S 31 References 32 Ordering Information 33 QIAGEN …
I used the QIAquick Gel Extraction Kit and followed the protocol supplied with the kit. However, I added 10ul of sodium acetate, 3M, pH 5.2, to the mixture of solubilised agarose and QG buffer
MinElute Gel Extraction Kit Protocols using a microcentrifuge 23 using a vacuum manifold 25 MinElute Reaction Cleanup Kit Protocols using a microcentrifuge 28 using a vacuum manifold 30 Troubleshooting Guide 32 Appendix: QIAvac Vacuum Manifolds 34 References 36 Ordering Information 37 1051747_HB 15.04.2008 13:24 Uhr Seite 3. 4 MinElute Handbook 03/2008 Kit Contents MinElute PCR Purification
Complete Genomics data is for Research Use Only and not for use in the treatment or diagnosis of any human subject. Information, descriptions and specifications in this publication are subject to …
WWW.QIAGEN.COM QIAvac 24 Plus Handbook QIAquick Gel Extraction Kits – QIAquick Nucleotide Removal Kits – MinElute PCR Purification Kits – MinElute Gel Extraction Kits – MinElute Reaction Cleanup Kits – Total RNA purification RNeasy Mini Kits VacConnectors* M13 ssDNA purification QIAprep Spin M13 Kit – * Optional accessory to prevent cross-contamination. QIAvac 24 Plus Handbook
The ultrafast purification protocol, based on the remarkable selectivity of patented QIAGEN Resin, allows the isolation of ultrapure, supercoiled plasmid DNA with high yields in less than one hour, and without centrifugation.
Quick-Start Protocols Show all products Show less QIAEX II Gel Extraction Kit (EN) Print Bookmark Share pdf . 48KB. English. Format File size Language Download Get Adobe Reader
Qiagen Minelute Gel Extraction Kit Protocol The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions.
QIAquick Gel Extraction Kit Protocol For more details, trouble-shooting, etc. please see Qiagen manual 1. Excise DNA fragment from agarose gel with a clean, sharp scalpel.
Qproteome Cell Compartment Handbook 04/2005 7 Introduction Eukaryotic cells are complex, well-ordered, and highly structured systems. The Cell Compartment Kit is designed for fast and easy subcellular fractionation of

QIAquick Gel Extraction Kit Sample to Insight – QIAGEN

Why is the concentration of DNA very low after a gel
In the Tilburg laboratory, the Qiagen columns (QIAamp DNA mini kit; Qiagen, Hilden, Germany) have been used for the extraction of DNA from sputum samples or BAL samples, to be tested by the Legionella PCR/probe assay. Negative controls, consisting of water (guaranteed DNA and RNA free; Sigma), were processed after every four clinical samples. At one point, it was found that some of the
gel using the QIAquick Gel Extraction Kit (Qiagen Iberia, Madrid, Spain). from E. coli DH-5α by usage of QIAprep Spin Miniprep and Midiprep kits (Qiagen, Hilden, labeled oligonucleotide extension, a rapid and quantitative protocol.
Wheeler Lab Protocols DNA Extraction – Qiagen DNeasy kit. Note the number of spin columns you use. The centrifuge can hold up to 30 samples at once.
Extraction of DNA fragments from polyacrylamide gels using the QIAquick® Gel Extraction Kit This procedure has been adapted from the QIAquick® Gel Extraction Kit Protocol and from Sambrook et al., 1989. It has not been thoroughly tested and optimized by QIAGEN. Please be sure to read the QIAquick Spin Handbook and the detailed QIAquick Gel Extraction Kit Protocol carefully before …
and print the MSDS for each QIAGEN kit and kit component. 24-hour emergency information Emergency medical information in English, French, and German can be obtained 24 hours a day from: Poison Information Center Mainz, Germany Tel: +49-6131-19240 . 6 Qproteome Nuclear Protein Handbook 07/2005 Introduction The functional architecture of the eukaryotic cell nucleus is of great interest to …
3 HokkaidoSystem Science.,Ltd. LaboPass TM GEL Introduction LaboPass TM Gel kit is designed for a fast and efficient isolation of 100 bp to 10 kb of DNA fragments from TAE or TBE agarose gel.

The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures “Spin column handling options A, B, C,
Pcr Purification Kit Qiagen Manual >>>CLICK HERE<<< It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin® Gel and PCR Clean-up kit is used for the first time. in this protocol with reagents from NEB and Finnzymes, except for the adapter (c) Purify on a QIAquick PCR cleanup column (QIAquick PCR Purification Kit. DNA purification · Manual
Qiagen Gel Extraction Protocol Pdf (EN) – QIAquick Gel Extraction Kit. Print Bookmark Share · more. pdf. 65KB. English. Format. File size. Language.
This often involves agarose gel electrophoresis to separate mixtures of DNA fragments, followed by extraction of the DNA fragment of interest from the gel. The importance of this step is obvious from the fact that every vendor of molecular-biology products produces a gel extraction kit. Most of these kits utilize a chaotropic agent, such as sodium iodide, to destabilize the agarose gel. The
Weigh the gel fragment in a colorless tube, and add 3 volumes of Buffer QG from the MinElute Gel Extraction Kit to the gel slice. 12. Let the gel slice completely dissolve in the Buffer QG at room temperature (~10 min).

Gel SV kit is designed for a fast and efficient isolation of 80 bp to 10 kb of DNA fragments from standard or low-melting agarose gel in TAE or TBE buffer system. Purified DNA can be directly used in ligation, labelling, sequencing and many other
PCR clean-up, gel extraction 1 Components 1.1 Kit contents • ®Several support protocols extend the application range of NucleoSpin Gel and PCR Clean-up to – Clean-up of DNA from reaction mixtures containing SDS (section 5.5) – Clean-up of single stranded DNA (section 5.6) – Extraction of RNA from agarose gels (section 5.4) – Extraction of DNA from polyacrylamide gels (section 5.3
RNeasy® Mini Handbook RNeasy Mini Kit For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup RNeasy Protect Mini Kit For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification RNeasy Plant Mini Kit For purification of total RNA from plants and filamentous fungi Fourth Edition September 2010 WWW.QIAGEN
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations.
Kit (12) Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels QIAamp RNA Blood Mini Kit — for total RNA purification from blood and body fluids
The E.Z.N.A.® Gel Extraction Kit is available with two different types of columns. V-spin V-spin columns have an attached cap, while Q-spin columns are capless.
HiPerFect Transfection Reagent QIAGEN Online Shop
QIAquick Gel Extraction Kit Protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 Troubleshooting Guide 28 Appendix: QIAvac Vacuum Manifolds 30 Handling Guidelines for QIAvac 6 31 QIAvac 24 32 Ordering Information 33 QIAGEN Companies and Distributors 35 QIAquick Spin Handbook 07/2002 3. Kit Contents QIAquick PCR Purification Kits (50) (250) Catalog No. 28104 28106 …
Molecular grade isopropanol Qiagen gel extraction kit Big Dye Terminator ready reaction mix v3.1 125 mM EDTA 100% ethanol 70% ethanol Hi Di formamide 3.3 Equipment Vortex mixer Automated sequencer and reagents Micro-centrifuge Pipettes and tips Freezer -18°C or lower Thermal cycler . OIE/FAO international reference laboratory for AI OneStep RT PCR for detection of H5 & H7 avian …
QIAEX II Agarose Gel Extraction Protocol This protocol is designed for the extraction of 40-bp to 50-kb DNA fragments from 0.3–2% standard or low-melt agarose gels in TAE or TBE buffers.
purified with QIAquick gel extraction kit (QIAGEN) and cloned. Packaging Protocol for Pooled Lentiviral shRNA Libraries. QIAquick Gel Extraction Kit (QIAGEN, Cat.# 28706).
gel extracted using the QIAquick Gel Extraction Kit (Qiagen) and clone. and immediate results and performance is comparable to the manual n Continue to use proven QIAGEN spin-column kits. QIAquick Gel
QIAquick Gel Extraction Kits for extraction of DNA fragments (70 bp – 10 kb) from standard, or low-melt agarose gels in TAE (Tris·acetate/EDTA) or TBE (Tris·borate/ EDTA) buffer and DNA cleanup from enzymatic reactions. – chapter 3 network protocols and communications pdf Gel extraction (Qiagen QIAquick GEL extraction kit) Materials: Gel containing DNA to extract QIAquick spin column Buffer PE (750 μl) Protocol: 1. Use gel extraction tips to extract the DNA band from the gel. Fit the tip around the band, push in, wiggle, and take out. …

QIAEX II Gel Extraction Kit (EN) QIAGEN

TM Tribioscience

LaboPass TM Gel hssnet.co.jp

Extraction of DNA from Agarose Gels SpringerLink

User-Developed Protocol Extraction of DNA fragments from

QIAquick Gel Extraction Kit Qiagen

Qiagen DNA Extraction Kits for Sample Preparation for

Qproteome Nuclear Protein Handbook Wolfson Centre Home
– HiSpeed™ Plasmid Purification Handbook Patel Lab
One Step PCR for detection of H5 and H7 avian influenza

E.Z.N.A.® Gel Extraction Kit – Omega Bio-tek

Identifying the m6A Methylome by Affinity Purification and

QIAEX II Handbook QIAEX II Agarose Gel Extraction Protocol
HiSpeed™ Plasmid Purification Handbook Patel Lab

Gel SV kit is designed for a fast and efficient isolation of 80 bp to 10 kb of DNA fragments from standard or low-melting agarose gel in TAE or TBE buffer system. Purified DNA can be directly used in ligation, labelling, sequencing and many other
The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures “Spin column handling options A, B, C,
Qproteome Cell Compartment Handbook 04/2005 7 Introduction Eukaryotic cells are complex, well-ordered, and highly structured systems. The Cell Compartment Kit is designed for fast and easy subcellular fractionation of
3 HokkaidoSystem Science.,Ltd. LaboPass TM GEL Introduction LaboPass TM Gel kit is designed for a fast and efficient isolation of 100 bp to 10 kb of DNA fragments from TAE or TBE agarose gel.

LaboPass TM Gel hssnet.co.jp
Qiagen DNA Extraction Kits for Sample Preparation for

The ultrafast purification protocol, based on the remarkable selectivity of patented QIAGEN Resin, allows the isolation of ultrapure, supercoiled plasmid DNA with high yields in less than one hour, and without centrifugation.
gel extracted using the QIAquick Gel Extraction Kit (Qiagen) and clone. and immediate results and performance is comparable to the manual n Continue to use proven QIAGEN spin-column kits. QIAquick Gel
In the Tilburg laboratory, the Qiagen columns (QIAamp DNA mini kit; Qiagen, Hilden, Germany) have been used for the extraction of DNA from sputum samples or BAL samples, to be tested by the Legionella PCR/probe assay. Negative controls, consisting of water (guaranteed DNA and RNA free; Sigma), were processed after every four clinical samples. At one point, it was found that some of the
Kit (12) Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels QIAamp RNA Blood Mini Kit — for total RNA purification from blood and body fluids
QIAquick Gel Extraction Kit Protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 Troubleshooting Guide 28 Appendix: QIAvac Vacuum Manifolds 30 Handling Guidelines for QIAvac 6 31 QIAvac 24 32 Ordering Information 33 QIAGEN Companies and Distributors 35 QIAquick Spin Handbook 07/2002 3. Kit Contents QIAquick PCR Purification Kits (50) (250) Catalog No. 28104 28106 …
the QIAamp® Viral RNA Mini Kit. support the extraction protocol as described below (70 µL input volume), an initial Qiagen Buffer AVL with carrier RNA is stable at …
PCR clean-up, gel extraction 1 Components 1.1 Kit contents • ®Several support protocols extend the application range of NucleoSpin Gel and PCR Clean-up to – Clean-up of DNA from reaction mixtures containing SDS (section 5.5) – Clean-up of single stranded DNA (section 5.6) – Extraction of RNA from agarose gels (section 5.4) – Extraction of DNA from polyacrylamide gels (section 5.3
3 HokkaidoSystem Science.,Ltd. LaboPass TM GEL Introduction LaboPass TM Gel kit is designed for a fast and efficient isolation of 100 bp to 10 kb of DNA fragments from TAE or TBE agarose gel.
MinElute Gel Extraction Kit Protocols using a microcentrifuge 23 using a vacuum manifold 25 MinElute Reaction Cleanup Kit Protocols using a microcentrifuge 28 using a vacuum manifold 30 Troubleshooting Guide 32 Appendix: QIAvac Vacuum Manifolds 34 References 36 Ordering Information 37 1051747_HB 15.04.2008 13:24 Uhr Seite 3. 4 MinElute Handbook 03/2008 Kit Contents MinElute PCR Purification
QIAEX II Agarose Gel Extraction Protocol This protocol is designed for the extraction of 40-bp to 50-kb DNA fragments from 0.3–2% standard or low-melt agarose gels in TAE or TBE buffers.
The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures “Spin column handling options A, B, C,
Pcr Purification Kit Qiagen Manual >>>CLICK HERE<<< It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin® Gel and PCR Clean-up kit is used for the first time. in this protocol with reagents from NEB and Finnzymes, except for the adapter (c) Purify on a QIAquick PCR cleanup column (QIAquick PCR Purification Kit. DNA purification · Manual

DNA Extraction – Qiagen DNeasy kit. research.amnh.org
E.Z.N.A.® Gel Extraction Kit Omega Bio-tek

18/11/2009 · One half was purified using four Qiagen gel extraction columns and the other half was purified using a single glass syringe filter as described above. The quantity of DNA purified using the glass syringe filter was equivalent to that purified by the four Qiagen gel extraction columns ( Figure. 5 ).
The ultrafast purification protocol, based on the remarkable selectivity of patented QIAGEN Resin, allows the isolation of ultrapure, supercoiled plasmid DNA with high yields in less than one hour, and without centrifugation.
Pcr Purification Kit Qiagen Manual >>>CLICK HERE<<< It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin® Gel and PCR Clean-up kit is used for the first time. in this protocol with reagents from NEB and Finnzymes, except for the adapter (c) Purify on a QIAquick PCR cleanup column (QIAquick PCR Purification Kit. DNA purification · Manual
MinElute Gel Extraction Kit Protocols using a microcentrifuge 23 using a vacuum manifold 25 MinElute Reaction Cleanup Kit Protocols using a microcentrifuge 28 using a vacuum manifold 30 Troubleshooting Guide 32 Appendix: QIAvac Vacuum Manifolds 34 References 36 Ordering Information 37 1051747_HB 15.04.2008 13:24 Uhr Seite 3. 4 MinElute Handbook 03/2008 Kit Contents MinElute PCR Purification
QIAquick Gel Extraction Kits for extraction of DNA fragments (70 bp – 10 kb) from standard, or low-melt agarose gels in TAE (Tris·acetate/EDTA) or TBE (Tris·borate/ EDTA) buffer and DNA cleanup from enzymatic reactions.
Weigh the gel fragment in a colorless tube, and add 3 volumes of Buffer QG from the MinElute Gel Extraction Kit to the gel slice. 12. Let the gel slice completely dissolve in the Buffer QG at room temperature (~10 min).
and print the MSDS for each QIAGEN kit and kit component. 24-hour emergency information Emergency medical information in English, French, and German can be obtained 24 hours a day from: Poison Information Center Mainz, Germany Tel: 49-6131-19240 . 6 Qproteome Nuclear Protein Handbook 07/2005 Introduction The functional architecture of the eukaryotic cell nucleus is of great interest to …
the QIAamp® Viral RNA Mini Kit. support the extraction protocol as described below (70 µL input volume), an initial Qiagen Buffer AVL with carrier RNA is stable at …
DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s Gel Extraction Kit and a Company Q kit for gel extraction following manufacturer’s recommended protocols.
Extraction of DNA fragments from polyacrylamide gels using the QIAquick® Gel Extraction Kit This procedure has been adapted from the QIAquick® Gel Extraction Kit Protocol and from Sambrook et al., 1989. It has not been thoroughly tested and optimized by QIAGEN. Please be sure to read the QIAquick Spin Handbook and the detailed QIAquick Gel Extraction Kit Protocol carefully before …
Molecular grade isopropanol Qiagen gel extraction kit Big Dye Terminator ready reaction mix v3.1 125 mM EDTA 100% ethanol 70% ethanol Hi Di formamide 3.3 Equipment Vortex mixer Automated sequencer and reagents Micro-centrifuge Pipettes and tips Freezer -18°C or lower Thermal cycler . OIE/FAO international reference laboratory for AI OneStep RT PCR for detection of H5 & H7 avian …
purified with QIAquick gel extraction kit (QIAGEN) and cloned. Packaging Protocol for Pooled Lentiviral shRNA Libraries. QIAquick Gel Extraction Kit (QIAGEN, Cat.# 28706).
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations.

Qiagen DNA Extraction Kits for Sample Preparation for
One Step PCR for detection of H5 and H7 avian influenza

Quick-Start Protocols Show all products Show less QIAEX II Gel Extraction Kit (EN) Print Bookmark Share pdf . 48KB. English. Format File size Language Download Get Adobe Reader
Weigh the gel fragment in a colorless tube, and add 3 volumes of Buffer QG from the MinElute Gel Extraction Kit to the gel slice. 12. Let the gel slice completely dissolve in the Buffer QG at room temperature (~10 min).
MinElute Gel Extraction Kit Protocols 19 using a microcentrifuge 19 using a vacuum manifold 21 MinElute Reaction Cleanup Kit Protocols 24 using a microcentrifuge 24 using a vacuum manifold 26 Troubleshooting Guide 28 Appendix: QIAvac Vacuum Manifolds 30 Handling Guidelines for QIAvac 24 30 Handling Guidelines for QIAvac 6S 31 References 32 Ordering Information 33 QIAGEN …
The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures “Spin column handling options A, B, C,

Extraction of DNA from Agarose Gels SpringerLink
QIAquick Gel Extraction Kit Sample to Insight – QIAGEN

the QIAamp® Viral RNA Mini Kit. support the extraction protocol as described below (70 µL input volume), an initial Qiagen Buffer AVL with carrier RNA is stable at …
RNeasy® Mini Handbook RNeasy Mini Kit For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup RNeasy Protect Mini Kit For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification RNeasy Plant Mini Kit For purification of total RNA from plants and filamentous fungi Fourth Edition September 2010 WWW.QIAGEN
Qiagen Minelute Gel Extraction Kit Protocol The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions.
Molecular grade isopropanol Qiagen gel extraction kit Big Dye Terminator ready reaction mix v3.1 125 mM EDTA 100% ethanol 70% ethanol Hi Di formamide 3.3 Equipment Vortex mixer Automated sequencer and reagents Micro-centrifuge Pipettes and tips Freezer -18°C or lower Thermal cycler . OIE/FAO international reference laboratory for AI OneStep RT PCR for detection of H5 & H7 avian …
18/11/2009 · One half was purified using four Qiagen gel extraction columns and the other half was purified using a single glass syringe filter as described above. The quantity of DNA purified using the glass syringe filter was equivalent to that purified by the four Qiagen gel extraction columns ( Figure. 5 ).
Qproteome Cell Compartment Handbook 04/2005 7 Introduction Eukaryotic cells are complex, well-ordered, and highly structured systems. The Cell Compartment Kit is designed for fast and easy subcellular fractionation of
QIAquick Gel Extraction Kit Protocol For more details, trouble-shooting, etc. please see Qiagen manual 1. Excise DNA fragment from agarose gel with a clean, sharp scalpel.
Complete Genomics data is for Research Use Only and not for use in the treatment or diagnosis of any human subject. Information, descriptions and specifications in this publication are subject to …
Extraction of DNA fragments from polyacrylamide gels using the QIAquick® Gel Extraction Kit This procedure has been adapted from the QIAquick® Gel Extraction Kit Protocol and from Sambrook et al., 1989. It has not been thoroughly tested and optimized by QIAGEN. Please be sure to read the QIAquick Spin Handbook and the detailed QIAquick Gel Extraction Kit Protocol carefully before …
QIAquick Gel Extraction Kits for extraction of DNA fragments (70 bp – 10 kb) from standard, or low-melt agarose gels in TAE (Tris·acetate/EDTA) or TBE (Tris·borate/ EDTA) buffer and DNA cleanup from enzymatic reactions.
MinElute Gel Extraction Kit Protocols 19 using a microcentrifuge 19 using a vacuum manifold 21 MinElute Reaction Cleanup Kit Protocols 24 using a microcentrifuge 24 using a vacuum manifold 26 Troubleshooting Guide 28 Appendix: QIAvac Vacuum Manifolds 30 Handling Guidelines for QIAvac 24 30 Handling Guidelines for QIAvac 6S 31 References 32 Ordering Information 33 QIAGEN …
Gel extraction (Qiagen QIAquick GEL extraction kit) Materials: Gel containing DNA to extract QIAquick spin column Buffer PE (750 μl) Protocol: 1. Use gel extraction tips to extract the DNA band from the gel. Fit the tip around the band, push in, wiggle, and take out. …
and print the MSDS for each QIAGEN kit and kit component. 24-hour emergency information Emergency medical information in English, French, and German can be obtained 24 hours a day from: Poison Information Center Mainz, Germany Tel: 49-6131-19240 . 6 Qproteome Nuclear Protein Handbook 07/2005 Introduction The functional architecture of the eukaryotic cell nucleus is of great interest to …
I used the QIAquick Gel Extraction Kit and followed the protocol supplied with the kit. However, I added 10ul of sodium acetate, 3M, pH 5.2, to the mixture of solubilised agarose and QG buffer

User-Developed Protocol Extraction of DNA fragments from
LaboPass TM Gel hssnet.co.jp

Extraction of DNA fragments from polyacrylamide gels using the QIAquick® Gel Extraction Kit This procedure has been adapted from the QIAquick® Gel Extraction Kit Protocol and from Sambrook et al., 1989. It has not been thoroughly tested and optimized by QIAGEN. Please be sure to read the QIAquick Spin Handbook and the detailed QIAquick Gel Extraction Kit Protocol carefully before …
In the Tilburg laboratory, the Qiagen columns (QIAamp DNA mini kit; Qiagen, Hilden, Germany) have been used for the extraction of DNA from sputum samples or BAL samples, to be tested by the Legionella PCR/probe assay. Negative controls, consisting of water (guaranteed DNA and RNA free; Sigma), were processed after every four clinical samples. At one point, it was found that some of the
QIAquick Gel Extraction Kit Protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 Troubleshooting Guide 28 Appendix: QIAvac Vacuum Manifolds 30 Handling Guidelines for QIAvac 6 31 QIAvac 24 32 Ordering Information 33 QIAGEN Companies and Distributors 35 QIAquick Spin Handbook 07/2002 3. Kit Contents QIAquick PCR Purification Kits (50) (250) Catalog No. 28104 28106 …
Qproteome Cell Compartment Handbook 04/2005 7 Introduction Eukaryotic cells are complex, well-ordered, and highly structured systems. The Cell Compartment Kit is designed for fast and easy subcellular fractionation of
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations.
Molecular grade isopropanol Qiagen gel extraction kit Big Dye Terminator ready reaction mix v3.1 125 mM EDTA 100% ethanol 70% ethanol Hi Di formamide 3.3 Equipment Vortex mixer Automated sequencer and reagents Micro-centrifuge Pipettes and tips Freezer -18°C or lower Thermal cycler . OIE/FAO international reference laboratory for AI OneStep RT PCR for detection of H5 & H7 avian …
QIAquick Gel Extraction Kits for extraction of DNA fragments (70 bp – 10 kb) from standard, or low-melt agarose gels in TAE (Tris·acetate/EDTA) or TBE (Tris·borate/ EDTA) buffer and DNA cleanup from enzymatic reactions.
WWW.QIAGEN.COM QIAvac 24 Plus Handbook QIAquick Gel Extraction Kits – QIAquick Nucleotide Removal Kits – MinElute PCR Purification Kits – MinElute Gel Extraction Kits – MinElute Reaction Cleanup Kits – Total RNA purification RNeasy Mini Kits VacConnectors* M13 ssDNA purification QIAprep Spin M13 Kit – * Optional accessory to prevent cross-contamination. QIAvac 24 Plus Handbook
DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s Gel Extraction Kit and a Company Q kit for gel extraction following manufacturer’s recommended protocols.